{"id":3499,"date":"2026-04-17T17:16:30","date_gmt":"2026-04-17T09:16:30","guid":{"rendered":"https:\/\/www.atlatl.center\/?page_id=3499"},"modified":"2026-04-22T17:25:33","modified_gmt":"2026-04-22T09:25:33","slug":"facs-sorting-center","status":"publish","type":"page","link":"https:\/\/www.atlatl.center\/fr\/platforms\/facs-sorting-center\/","title":{"rendered":"FACS & Centre de Tri"},"content":{"rendered":"
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FACS & Centre de Tri<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Le centre FACS & Sorting d'ATLATL est con\u00e7u pour aider les \u00e9quipes \u00e0 analyser, isoler et valider rapidement et pr\u00e9cis\u00e9ment des populations cellulaires cl\u00e9s.<\/span><\/p>

En combinant la cytom\u00e9trie en flux multiparam\u00e9trique avec le tri cellulaire st\u00e9rile en syst\u00e8me clos, la plateforme permet une caract\u00e9risation et une isolation pr\u00e9cises des cellules \u00e0 partir d'\u00e9chantillons complexes tels que le sang, les tissus et les syst\u00e8mes cellulaires modifi\u00e9s. Cela permet aux chercheurs de g\u00e9n\u00e9rer des donn\u00e9es de haute qualit\u00e9, pr\u00eates \u00e0 la d\u00e9cision pour des applications en aval.<\/p>

Situ\u00e9s dans les bio-hubs d'ATLATL, les scientifiques travaillent c\u00f4te \u00e0 c\u00f4te avec des experts techniques, permettant une mise en place rapide des essais, une optimisation en temps r\u00e9el et une ex\u00e9cution efficace. La plateforme prend en charge un large \u00e9ventail d'applications, de la biologie cellulaire de base aux programmes avanc\u00e9s de th\u00e9rapie cellulaire et d'immunologie, acc\u00e9l\u00e9rant ainsi la recherche et le d\u00e9veloppement translationnel.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Capacit\u00e9s de base :<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Analyse Cellulaire Multiparam\u00e9trique<\/b><\/p>

Analyse \u00e0 haut d\u00e9bit du ph\u00e9notype, de la fonction et de la viabilit\u00e9 cellulaires dans des populations complexes.<\/p>

Tri cellulaire (syst\u00e8me ferm\u00e9)<\/b><\/p>

Isolement st\u00e9rile et de haute puret\u00e9 des populations cellulaires cibles gr\u00e2ce \u00e0 une technologie de tri sans contamination bas\u00e9e sur des cartouches, adapt\u00e9e aux applications en aval sensibles.<\/p>

Immunoph\u00e9notypage<\/b><\/p>

Profilage d\u00e9taill\u00e9 des populations de cellules immunitaires (cellules T, cellules B, cellules NK, etc.) \u00e0 l'aide de marqueurs de surface et intracellulaires.<\/p>

Tests cellulaires fonctionnels<\/b><\/p>

\u00c9valuation de la production de cytokines, de la prolif\u00e9ration, de l'apoptose, des voies de signalisation et d'autres fonctions cellulaires.<\/p>

Cycle cellulaire et analyse de viabilit\u00e9<\/b><\/p>

Mesure de la progression du cycle cellulaire, de la prolif\u00e9ration et de l'apoptose pour validation exp\u00e9rimentale.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u00c9quipements :<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Analyseur MACSQuant X de Miltenyi Biotec<\/p>

Miltenyi Biotec MACSQuant\u00ae Analyzer 10<\/p>

Miltenyi Biotec MACSQuant\u00ae Analyzer 16<\/p>

Miltenyi Biotec MACSQuant\u00ae Analyzer VYB<\/p>

Miltenyi Biotec MACSQuant\u00ae Tyto<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\"Miltenyi<\/figure><\/div>
\"analyseur<\/figure><\/div>
\"Analyseur<\/figure><\/div>
\"miltenyi<\/figure><\/div>
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Analyse FACS<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Le support d\u00e9taill\u00e9 des canaux de fluorescence de notre centre de tri cellulaire et de FACS est list\u00e9 ci-dessous.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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La plateforme MACSQuant Analyzer de notre plateforme principale comprend quatre instruments (configurations X, VYB, 10 et 16), offrant un support polyvalent pour un large \u00e9ventail d'essais, y compris l'immunoph\u00e9notypage et l'analyse fonctionnelle de cellules marqu\u00e9es par fluorescence. Elle convient parfaitement aux chercheurs dans les domaines de la fabrication de cellules, de l'immunologie, de la biologie des syst\u00e8mes, de la biologie cellulaire, des neurosciences et des domaines connexes, offrant une analyse cytom\u00e9trique en flux tr\u00e8s fiable et sensible avec un minimum de temps de manipulation.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Tri FACS<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Le MACSQuant\u00ae Tyto\u00ae est un trieur de cellules de paillasse de nouvelle g\u00e9n\u00e9ration \u00e9quip\u00e9 de 3 lasers, permettant le tri de cellules \u00e0 10 param\u00e8tres. Une caract\u00e9ristique unique de l'instrument est le fait que le processus de tri r\u00e9el se d\u00e9roule exclusivement dans la cartouche MACSQuant Tyto. La cartouche fournit un syst\u00e8me \u00e0 usage unique, jetable et enti\u00e8rement ferm\u00e9, \u00e9liminant le risque de contamination et de contamination crois\u00e9e des \u00e9chantillons.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Applications R&D :<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Th\u00e9rapie cellulaire et programmes CGT<\/b>
Isolation et validation des cellules modifi\u00e9es (par exemple, CAR-T, cellules souches)<\/p>

Immunologie et \u00e9tudes de l'inflammation<\/b>
Profilage immunitaire et essais fonctionnels<\/p>

Validation de la cible<\/b>
Identifier et isoler les populations cellulaires pertinentes<\/p>

Soutien au d\u00e9pistage de drogues<\/b>
Analyses des r\u00e9ponses et des donn\u00e9es fonctionnelles bas\u00e9es sur les cellules<\/p>

D\u00e9couverte de biomarqueurs<\/b>
Identification des sous-ensembles cellulaires pertinents pour la maladie<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

FACS & Sorting Center The FACS & Sorting Center at ATLATL is designed to help teams analyze, isolate, and validate key cell populations quickly and accurately. By combining multiparametric flow cytometry with sterile, closed-system cell sorting, the platform enables precise […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":752,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","site-sidebar-layout":"left-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"normal-width-container","site-content-style":"default","site-sidebar-style":"boxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"659","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-3499","page","type-page","status-publish"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/pages\/3499","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/comments?post=3499"}],"version-history":[{"count":10,"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/pages\/3499\/revisions"}],"predecessor-version":[{"id":3986,"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/pages\/3499\/revisions\/3986"}],"up":[{"embeddable":true,"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/pages\/752"}],"wp:attachment":[{"href":"https:\/\/www.atlatl.center\/fr\/wp-json\/wp\/v2\/media?parent=3499"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}